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Keywords:
Breast, Oncology, Animal (veterinary) studies, CT, Image manipulation / Reconstruction, Computer Applications-General, Experimental investigations, Outcomes analysis, Cancer, Biological effects
Authors:
S. Kossatz, M. Stapf, R. Ludwig, H. Dähring, I. Hilger; Jena/DE
DOI:
10.1594/ecr2014/C-0174
Methods and materials
We used athymic nude mice bearing subcutaneously implanted breast cancer xenografts derived from the cell line MDA-MB-231.
MNP were produced by Liquids Research Limited (Bangor,
UK) by means of the co-precipitation technique [2] and were coated with dimercapotsuccinymid acid.
Intratumoral injection of MNP was performed by slow bolus injection of 0.25 mg Fe / 100 mm3 tumor.
Mice were then imaged using a microCT Scanner (TomoScope Synergy Twin,
CT-Imaging GmbH,
Erlangen,
Germany) using a low radiation dose protocol (29 s,
65 kV) under isoflurane anesthesia.
MNP distribution and volume were analyzed with the Imalytics Research Software (Philips Technologie GmbH,
Aachen,
Germany).
For hyperthermia treatment,
the tumor of anesthetized animals was placed inside the coil of an alternating magnetic field (AMF,
H = 15.4 kA/m,
f = 435 kHz) for 60 min and tumor surface temperature was additionally monitored using an infrared thermography camera.
Photographs of animals were taken before and two days after magnetic hyperthermia treatment.