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Keywords:
Contrast agent-intravenous, CT, Molecular imaging, Biological effects
Authors:
I. Willekens, N. Buls, T. Lahoutte, F. Verhelle, J. de Mey; Brussels/BE
DOI:
10.1594/ecr2015/C-2448
Methods and materials
Healthy C57bl/6 mice (n=2) were used.
Anesthesia was induced with 5% isoflurane and maintained at 2% during the scan with spontaneous breathing via a mask.
Aurovist (Nanoprobes,
New York,
USA) was administered intravenously at a dose of 200 µl.
Aurovist is a contrast agent containing gold nanoparticles.
Imaging was performed using micro-CT (SkyScan 1178 micro-CT system; Bruker MicroCT,
Kontich,
Belgium) at a resolution of 83 µm.
The total acquisition time was 6 min.
Each animal underwent a micro-CT scan before contrast injection,
immediately after contrast injection and at 1h,
2h,
3h,
4h,
6h,
24h and 48h after contrast.
Images were reconstructed using filtered backprojection and analysed using Amide.
Regions of interest (ROIs) were drawn in spleen,
liver,
and left ventricle.
The contrast enhancement was measured and expressed in function of time.