Keywords:
Performed at one institution, Diagnostic or prognostic study, Prospective, Osteoporosis, Demineralisation-Bone, Blood, Screening, Absorptiometry / Bone densitometry, Trauma, Musculoskeletal spine, Bones, Musculoskeletal
Authors:
�. Bartha, A. Zubrecki, I. Devosa, A. Marodi, K. Gion, G. Bartfai; Szeged/HU
DOI:
10.26044/ecr2020/C-03953
Methods and materials
202 of Caucasian female between 40-80-year-old (mean: 63.72, SD: 7.97) participated in this survey between 2015 and 2017. Physical examination included height, weight measurement. Body mass index (BMI) was calculated with the following formula: BMI=weight (kg)/height (m2). BMD was measured in femoral neck (FN) and lumbar spine (LS; L1-4) using dual-energy X-ray absorptiometry (DXA; Lunar Prodigy Advance, GE Healthcare Malborough, MA, USA). The DXA result were analyzed by enCORE-W7 Software (version:16SP2) (GE Healthcare, Malborough, MA, USA). Body composition was also analyzed by DXA. We also searched our database and collected participants’ previous and follow up DXA LS and FN scores between 2005 and 2019.
The diagnosis of osteoporosis was defined according to the World Health Organization diagnostic classification criteria used by the result of FN and LS measurements by DXA.
Osteoporosis was defined as as BMD 2.5 standard deviations or more below the mean peak bone mass (healthy adult, age-, race- and sex-matched reference population). Obesity was determined as BMI≥25 kg/m2.
Self-reported questionnaire were used to determine alcohol consumption, smoking, dietary habits, medical illness and reproductive factors like number and age of delivery. Participants were divided into 2 groups based on the age of last delivery: before 30 year-old (before 30, n=130) and at 30-year-old or over it (over 30, n=70).
Serum analyses
Blood samples were collected for biochemical analyses from all subjects during the study. Blood samples were collected after overnight fast. Serum 25-hydroxivitamin D levels were measured by chemluminescens immunoassay (CLIA) as the manufacturer proposed (Liaison 25 OH Vitamin D TOTAL Assay and Liaison analyzer, DiaSorin Inc, Stillwater, MN, USA). Serum ß-CTx were measured by electrochemiluminescens immunoassay (ECLIA) as the manufacturer proposed (Elecsys ꞵ-CrossLaps assay and Cobas 602 analyzer, Roche Diagnostics GmbH, Mannheim, Germany).
Statistical analysis
Data were determined for normal distribution. Values are peresented as mean± standard deviation (SD). Pearson correlation coefficient test and two-tailed Student t-test were used.