Keywords:
Gastrointestinal tract, MR, Experimental investigations, Obstruction / Occlusion
Authors:
F. Iacobellis, D. Berritto, F. Somma, M. P. Belfiore, S. Cappabianca, A. Rotondo, R. Grassi; Naples/IT
DOI:
10.1594/ecr2012/B-0199
Methods and Materials
Adult Sprague Dawley rats (n=8) were divided in 2 groups: in the first (n=4),
rats underwent a surgical procedure of small bowel ligation followed by 8 hrs macroscopical monitoring; in the latter (n=4),
7T micro-MR scannings were performed before and after SBO.
In both cases,
injured bowel was processed for histological analysis.
Rats were anesthetized with Ketamine (100 mg/kg im) and Medethomidine (0,25 mg/Kg im) injections and maintained under anesthesia during all the procedures; the body temperature was maintained at 37.0 ± 0.5 °C.
Group I rats (n=4),
underwent a laparotomic surgical procedure of SBO followed by 8 hrs macroscopical monitoring: snapshots of exposed bowel and mesentery were recorded at predetermined time points.
In Group II rats (n=4),
7T-microMR scans (Bruker BioSpec 70/16US; Bruker Medical Systems,
Ettlingen,
Germany) were performed before and after SBO with RareT2 sequence in axial section (TR 6060.3 ms; TE 36.0 ms; FOV 7.00 cm; IS 1.00/1.00 mm; N slice 52).
At the end of observation,
all rats were euthanized by an intrapulmonary injection of Tanax 0,5 ml and the large bowel excised for the histological analysis.