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Keywords:
Professional issues, Radiographers, Digital radiography, Conventional radiography, CT, Health policy and practice, Technical aspects, Technology assessment, Education and training, Occupational / Environmental hazards, Quality assurance
Authors:
A. Bras, K. B. Azevedo, A. F. Abrantes, L. P. Ribeiro, S. Rodrigues, A. M. Ribeiro, R. P. P. Almeida; Faro/PT
DOI:
10.1594/ecr2017/B-0830
Methods and materials
The amount of bacteria present is evaluated by counting the number of colony-forming units (CFU) in a 47mm petri dish.
It is possible to see an example of the petri dish and respective CFU over the sterile membrane in Fig. 1.
To access the CFU that may pass to the receiver, a sterile membrane were swabed,
with a sterile tweezer, in the medial sternum,
another in the epigastric region and another in the interscapular zone.
Then,
the sterile membre was placed in a sterile 47mm petri dish with a non-selective media (NSM) of soy triptone and then incubated.
To access the CFU that passed to the receiver, three 120mm sterile petri dish were placed in contact with the respective zones as explained above.
Then,
the petri dish was swabed with a sterile membrane with the help of a sterile tweezer,
then the sterile membre was placed in a sterile 47mm petri dish with NSM and then incubated.
To access the CFU effectivelly present in the patient, three sterile 47mm petri dish filled with NSM were placed in contact directly with the same respective zones as above and then incubated.
After the analysis of the above data,
to evaluate the types of bacteria present that can develop in the available selective medias,
swabs in petri dishes evaluating the CFU that may pass to receivers were done,
also with sterile membranes with the help of a sterile tweezer,
and placed in selective SB (for streptococcus),
Chapman (staphylococcus epidermis and aureus) and MacConkey (enterococcus and proteus) culture medias and then incubated.
All samples were incubated for 42 hours at 36ºC.