It was found high intra-evaluation reliability for colour mapping values by QSE ultrasound. In few cases, ICC was below the acceptable 0.70 levels. Vastus lateralis muscle was defined as a soft tissue, with higher percentage of green colour, followed by the blue colour when in rest. In generally, there were significant differences on red colour percentage between the first and second evaluations and between the rest and contraction, showing on these two evaluations a less percentage of red colour that is an increase on the tissue hardness after dynamic exercise.