Keywords:
Oncologic Imaging, CNS, MR, Imaging sequences, Neoplasia, Prospective, Diagnostic or prognostic study, Multicentre study
Authors:
C. Giordano1, S. Cottonaro2, P. Guadalupi3, A. Marrazzo3, S. Gaudino4, M. F. romano1, C. Colosimo4; 1Napoli/IT, 2Catania/IT, 3Roma/IT, 4Rome/IT
DOI:
10.26044/ecr2020/C-10493
Results
Figure 1 shows the morphological characteristics of the studied cases in MRI, a score was assigned to the necrotic aspect on a scale ranging from a minimum of 1 to a maximum of 3.The evaluation of the necrotic aspect at MR imaging was based on T2, T1 and T1 after contrast weighted images.
No differences were found between patients and controls in the CD4 and CD8 T-cell counts, both total and FKBP51s-expressing subsets. Although the CD4 FKBP51s subpopulation appears to be increased in patients compared to controls, this increase was not significan.
Total Treg and Treg FKBP51s counts show a significant increase in patients compared to control subjects. Of particular interest is the increase in the Treg FKBP51s count in patients who show positive immunohistochemistry for the PD-L1 marker, compared with patients in whom the PD-L1 signal is absent (Table I).
We found a significant increase in monocytes expressing the CD163 marker in patients compared to controls (Figure 3A). This marker is associated with an alternative polarization profile or M2 which characterizes monocytes with immunosuppressive activity. CD163 + monocytes, in GBM patients, expressed FKBP51s to varying degrees. Figure 3B shows the cytofluorimetric phenotypes of the monocytes of the cases represented in Figure 1. In the G2 phenotype, the ratio between the FKBP51s + fraction and the FKBP51s- fraction (which we define as FKBP51s index) is 0.2. For the G1 patient, this index is 1.95. In patient G8, the index 0.83.
A positive linear relationship was assessed between the FKBP51s index and the necrosis score (defined according to MR) (Figure 4)
Figure 5 shows the PRE and POST-surgery values of the CD163/FKBP51s subpopulation in 5 patients. In two cases (G3 and G11) there is a significant increase in this subpopulation after surgical removal of the tumor. MR reveals the presence of residual tumor in these patients.