Purpose
The present study aimed to quantitative evaluate changes in tumor microvessels using synchrotron radiation in vivo after intraarterial and intravenous administration of anticancer drugs.
Methods and Materials
1. Preparation of MaterialsVX2 carcinomas were transplanted into the auricles of 50 rabbits. Three days later, rabbits were divided into three groups. Group IA (n=20) was administered anticancer drugs by intraarterial injection over 5 min. Group DIV (n=20) was administered anticancer drugs by drip infusion over 15 min. The two groups were administrated cisplatin (n=10), carboplatin (n=10), docetaxel hydrate (n=10) and adriamycin (n=10), respectively. Untreated VX2 rabbits (n=10) that was administrated sodium saline served as the Control group. In all groups, tumor microvessels were microangiologically...
Results
In the control group, the number of tumor microvessels remained stationary during the experimental time, and there was no significant difference in the AOI between before and 30 min. afteradministration.Microangiograms of IA groupIA group (docetaxel hydrate) : The number of tumor microvessels had decreased after 5 min. remarkably. Even 30 min. later the microvessels were in a state of reduction. Changes in microvessels of approximately 50 to 200micron in diameter in the central zone of tumor were observed.Microangiograms of DIV groupDIV group (carboplatin) : The...
Conclusion
Based on the changes in the tumor microvessels that were observed, the effect of anticancer drugs upon the tumor microvessels or tumor cells (expect vasospasm associated with anticancer drugs and contrast media) was considered significant, because even 30min. later the tumor microvessels were in the state of reduction. The synchrotron radiation system was a useful tool for quantitative evaluation of changes in tumor microvessels after administration of anticancer drugs. We now intend to study quantitative evaluation of changes in tumor microvessels after administration of angiogenesis...
References
Umetani K, Yagi N, Suzuki Y, et al. Observation and analysis of microcirculation using high-spatial-resolution image detectors and synchrotron radiation. Soc Photo-Opt Instrum Eng 2000;3977:522-533Takenori Y, Imai S, Maehara N, et al. Experimental studies of the microangioarchitecture of tumors using synchrotron radiation at SPring-8. Nucl Instr Methods Phys Res A 2002;467/468:1346-1348
Personal Information
SHIGEKI IMAI.MD PHD Associate Professor Department of Diagnostic Radiology, Kawasaki Medical School577 Matsushima Kurashiki Okayama 701-0192 JAPANE-mail link:
[email protected]