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Keywords:
Multidisciplinary cancer care, Cancer, Comparative studies, MR-Spectroscopy, Oncology, Breast, Pathology
Authors:
S. Makkat1, A. Schiettecatte1, Y. Fierens2, K. De Pierre1, M. Carprieaux1, J. De Greve1, M. Vanhoey1, J. de Mey1; 1Brussels/BE, 2Jette/BE
DOI:
10.1594/ecr2015/C-2179
Methods and materials
The preliminary results concerning the first 23 patients with breast cancers (age range 47-74 years; mean 51 years) included in a prospective ongoing study,
are discussed here (Table 1).
Patients who had tumor of at least 1.5cm dimension in all the three planes and who underwent mastectomy or wide excision after MR imaging were studied by in-vivo MR mammography protocol including MRS on a 1.5 T scanner (Philips Intera,
Best Nederland).
The MRS technique was a PRESS single voxel sequence (TE 270 ms,
TR 2000ms).
Post-processing was done using jMRUI 4.0 which consisted of 5 Hz apodisation,
manual phase correction and zero filling.
The AMARES algorithm was used to quantify the tCho using water as the internal reference [2].
Operative tumor specimens were immunostained for Ki67 with MIB-1 antibody.
The definitive diagnosis was obtained histologically after excisional biopsy or mastectomy.
Formalin-fixed,
paraffin-embedded slides were reviewed by senior pathologists to evaluate histological size,
lymph node status and histological grade and Nottingham prognostic index.
Operative tumor specimens were immunostained for Ki67 with MIB-1 antibody [3].
Tumor samples were stained for estrogen receptor (ER),
progesterone receptor (PR) and HER-2 protein by immunohistochemistry (IHC).
Tumors were considered hormone receptor positive if a nuclear staining was observed in at least 5% of the tumor cells.
For the HER-2 protein,
the slides were scored as 0,
1+,
2+,
or 3+ according to DAKO guidelines.
For an HER-2 protein DAKO score of 2+ and 3+,
HER-2 gene status was assessed by fluorescence in-situ hybridization.
Estimated tCho values were correlated with tumor grades and Ki67 index.