Dynamic contrast enhanceded MRI in a xenotransplant model of giant cell arteritis

Congress:

ECR 2019

Poster Number:

C-3621

Type:

Scientific Exhibit

Keywords:

Inflammation, Experimental investigations, MR-Angiography, Vascular

Authors:

M. Both¹, M. Kaul², M. Salehi Ravesh¹, K. Syed¹, J. Humbert¹, O. Will¹, U. Schumacher², O. Jansen¹, S. Ullrich²; ¹Kiel/DE, ²Hamburg/DE

DOI:

10.26044/ecr2019/C-3621

DOI-Link:

https://dx.doi.org/10.26044/ecr2019/C-3621

Fig. 1: Human temporal artery specimen as a xenograft implanted to the subcutaneous...

Fig. 2: Dynamic contrast enhanced MRI of a subcutaneous arterial xenograft

Fig. 3: Time-intensity-curve during DCE in a xenograft and in the mouse muscle.

Aims and objectives

To evaluate the feasibility of a mouse xenograft transplantation model of giant cell arteritis as an vivo method for the assessment of contrast media perfusion in MRI.

Methods and materials

Temporal artery biopsy specimen (n=6) of patients with active giant cell arteritis were implanted subcutaneously into immunodeficient pfp/rag2(-/-) mice. After 3 weeks, a 0,15 mmol/kg bolus of gadolinium-diethylenetriaminepentaacetic acid (GD-DTPA) was administered via the tail vein. Dynamic contrast enhanced (DCE) MRI was performed using a T1 weighted gradient echo sequence (TR: 70ms, TE: 1,06 ms, FA: 20°) in a 7T animal MRI scanner (Clinscan 70/30 with syngo MR B15, Bruker Biospin GmbH, Ettlingen, Germany).

Results

Contrast enhancement as a predictor of sufficient ingrowth was found in 6/6 vascular xenografts. Time-intensity curve upslope was faster in the transplanted arteries than in the mouse muscel, and the mean area under the curve ratio was 1.6, indicating hyperperfusion of the temporal artery biopsy specimen.

Conclusion

To our knowledge, this is the first study to perform MRI with DCE in a murine xenotransplant model of giant cell arteritis. Revealing different perfusion pattern in the xenograft and in the mouse muscle, this is a promising in vivo method for DCE MRI studies in large vessel vasculitis. The procedure could be used to assess the effectiveness of new inflammatory drugs in giant cell arteritis.

Personal information

References

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