Aims and objectives
Stem cells transplantation for regenerative medicine has shown promising therapeutic results in various injury animal models and even in humans during first clinical trials [1].
Currently several clinical trials are being conducted worldwide,
showing the effectiveness of stem cells therapy in the treatment of such pathological conditions as ischemic stroke [1,2] spinal cord injury [3],
myocardial infarction [4] and disorders of the skeletal system [5].
Multiple studies have demonstrated that transplanted stem cells are capable of homing and engrafting into areas of tissue injury [6],...
Methods and materials
Mesenchymal stem cells culture and labeling
We have chosen hMSCs as they have been considered among the other types of stem cells the safest and promising for treating brain injury.
HMSCs were derived from human placenta and maintained in culture.
HMSCs were labeled with SPIO (Bangs Laboratories; diameter of 0.96 μm) consisting of a magnetic iron oxide core and a chemically inert shell containing the Dragon Green fluorescent dye (ex=480 nm,
em=520 nm).
The efficacy of labeling evaluated by flow cytometry exceeded 90% and remained...
Results
Using in vitro cellular MRI we found,
that on T2*flash2d MR-signal decreases proportionally to the increase of SPIO-labelled cell concentration (hence,
the amount of iron).
T2*w medic,
T2*w flash3d and SWI were extremely sensitive to magnetic susceptibility,
thus even the tube with the smallest amount of hMSCs (10 cells in 20 µl) wasn’t visualized.
Т2w image sequence was the least sensitive for labelled cells detection (figure 1).
On the base of obtained data further we used the minimal concentration of labelled cells in tissue-equivalent phantom....
Conclusion
In our study due to MRI and fluorescence histology we evaluate that SWI is more sensitive for tracing iron oxide labelled stem cells then T2*w flash3d,
T2*w medic,
T2*flash2d and T2w respectively.
SWI can be used in laboratory and clinical practice as a sensitive and high resolution MR-sequence for in vivo SPIO-labeled stem cells tracking.
Personal information
Daria Namestnikova,
M.D.,
assist.
prof.
Department of Fundamental and Clinical Neurology,
The Russian National Research Medical University named after N.I.Pirogov,
Moscow,
RF.
[email protected]
Ilya Gubsky,
M.D.
Russian Medical Academy of Postgraduate Education,
Department of Radiology,
Radiotherapy and Medical Physics,
Moscow,
RF.
[email protected]
Leonid Gubsky,
M.D.,
Dr.,
Prof.
Department of Fundamental and Clinical Neurology,
The Russian National Research Medical University named after N.I.Pirogov,
Moscow,
RF.
[email protected]
Irina Kholodenko,
Ph.D.
V.N.Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences,
Moscow,
RF.
[email protected]
Konstantin Yarygin,...
References
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Intravenous administration of autoserum-expanded autologous mesenchymal stem cells in stroke.
Brain 2011; 134 (10): 1790–1807.
Bang OY.,
Lee JS.,
Lee PH.
et al.
Autologous mesenchymal stem cell transplantation in stroke patients.
Ann Neurol 2005; 57:874–882.
Callera F.,
de Melo CM.
Magnetic resonance tracking of magnetically labeled autologous bone marrow CD34+ cells transplanted into the spinal cord via lumbar puncture technique in patients with chronic spinal cord injury: CD34 + cells’ migration into the injured site.
Stem Cells Dev 2007; 16:461–466.
Meyer GP,
Wollert...