This poster is published under an
open license. Please read the
disclaimer for further details.
Keywords:
Transplantation, Image verification, Grafts, Surgery, Contrast agent-other, MR, Experimental, Molecular imaging, CNS, Animal (veterinary) studies
Authors:
D. Namestnikova, I. L. Gubskiy, L. Gubsky, I. Kholodenko, K. Yarygin, K. Sukhinich, I. Vakhrushev, V. Pogoreltsev, M. Abakumov; Moscow/RU
DOI:
10.1594/ecr2015/C-2308
Results
Using in vitro cellular MRI we found,
that on T2*flash2d MR-signal decreases proportionally to the increase of SPIO-labelled cell concentration (hence,
the amount of iron).
T2*w medic,
T2*w flash3d and SWI were extremely sensitive to magnetic susceptibility,
thus even the tube with the smallest amount of hMSCs (10 cells in 20 µl) wasn’t visualized.
Т2w image sequence was the least sensitive for labelled cells detection (figure 1).
On the base of obtained data further we used the minimal concentration of labelled cells in tissue-equivalent phantom.
As shown in Figure 2,
the relative signal decrease in the areas with pronounced changes of magnetic susceptibility (SPIO-labelled cells) was observed in SWI (Fig.2,
scheme M).
Thus,
the best traceability of SPIO-labelled cells was obtained using SWI.
In vitro data was confirmed by in vivo MRI of intact rat brain.
As shown in figure 3 the minimal concentration of labelled cells is better visualized in SWI.
Histological analysis of brain slices confirmed that the MR-signal reduction areas are zones of labelled hMSCs accumulation (figure 4).
Comparing MRI data and histology we have shown that SWI can visualize labeled hMSC more sensitively,
with larger size and greater contrast than T2-,
T2*WI.